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Western Blotting: How to Optimize Your Signal to Noise

When Western blotting, it may be difficult to get a “clean” blot with a good signal to noise ratio. This video shows you how to optimize your Western blot results by determining the optimal concentration of secondary antibody.

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Operating an HPLC: Part 2

HPLC, or High Performance Liquid Chromatography, is an analytical tool used in laboratories to detect individual solutes within a complex mixture. This video provides an introduction on how to purge your HPLC with mobile phase (prime the system) and attach the column.

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Operating an HPLC: Part 1

HPLC, or High Performance Liquid Chromatography, is an analytical tool used in laboratories to detect individual compounds (solutes) within a complex mixture. Solutions called mobile phases are needed in HPLC.

This video provides an introduction on how to prepare your mobile phase by filtering it.