How to: Set-up automated two-step purification

Automated multistep protein purification can improve efficiency in protein purification workflows. The automation methods can vary from a simple two-step method to sophisticated methods with high level of automation. This video shows how to set up a two-step method in the UNICORN software for the ÄKTA pure protein purification system.

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Operating an HPLC: Part 2

HPLC, or High Performance Liquid Chromatography, is an analytical tool used in laboratories to detect individual solutes within a complex mixture. This video provides an introduction on how to purge your HPLC with mobile phase (prime the system) and attach the column.

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Operating an HPLC: Part 1

HPLC, or High Performance Liquid Chromatography, is an analytical tool used in laboratories to detect individual compounds (solutes) within a complex mixture. Solutions called mobile phases are needed in HPLC.

This video provides an introduction on how to prepare your mobile phase by filtering it.