How to: Set-up automated two-step purification

Automated multistep protein purification can improve efficiency in protein purification workflows. The automation methods can vary from a simple two-step method to sophisticated methods with high level of automation. This video shows how to set up a two-step method in the UNICORN software for the ÄKTA pure protein purification system.

Principles of Ion Exchange Chromatography

Ion exchange chromatography is one of the most frequently used techniques for purification of biomolecules and separates the molecules according to differences in their net surface charge. This video describes the principles of the technique.